Knockdown of EGFP expression. A.
Culture of myocytes infected with Ad-EGFP and the same amount of empty virus. Aa
, transmitted light at low magnification; Ac
, same field as fluorescence image. Ab
, phase contrast image of selected cells at higher magnification. The inset shows regular cross striation pattern at higher magnification. Ad
, fluorescence image of the same field. B.
Photomicrographs of a sister culture infected with Ad-EGFP plus Ad-siEGFP; a through d have the same meaning as in panel A. The cells were infected about 24 h after plating. Photomicrographs were taken on d 8 days after infection. C.
Time-dependence of siRNA-induced decrease in cell fluorescence. Bars represent mean fractions of fluorescent cells SE; n=3 i.e. 3 dishes containing myocytes isolated from different animals; 4 fields of 1 mm2 were counted and averaged. Ad-EGFP / Ad-siEGFP double infected cultures are represented by the open bars. Black bars represent Ad-EGFP / empty virus infected controls. D.
Time dependence of siRNA-induced reduction of EGFP mRNA (n=6).